Candover to sell CPL to its management

first_img CANDOVER Investments will spin off its private equity management arm to a group of its directors as part of its wider plan to wind down the firm. It will offload Candover Partners Limited (CPL) to new entity Arle Capital Partners, an investment advisory firm set up by the group of its executives, for a nominal sum and sell 29 per cent of its investment portfolio to an entity backed by Arle and private equity group Pantheon.The value of the sale is expected to come in at around £60m – a heavy discount to the carrying value of the assets. The funds will be returned to its investors as the firm seeks to claw back losses incurred during the financial crisis.Candover also announced long-standing chairman Gerry Grimstone plans to step down after the sale closes, adding “the search for a successor has begun”.Malcolm Fallen, Candover’s chief executive, said: “This is the last significant step in implementing our plans to return cash to shareholders over time.“The sale of CPL to its executives will create an independent, motivated and incentivised manager focused on maximising and realising the value in the portfolio.”Candover’s move to sell its portfolio of companies and return cash to investors follows a failed attempt to sell the business in August.The firm was undone by a series of ill-judged acquisitions at the height of the boom. But when the market went sour, Candover saw the net value of its assets fall by half and was forced to allow investors a chance to cancel outstanding commitments to the fund.The Candover Partners sale comes after an announcement in September that it had agreed the sale of its Equity Trust unit to private equity rival Doughty Hanson, in a deal valuing the business at £297m.It also said in October a deal to sell its Ontex nappy-making business to US buyout firm TPG and the private equity unit of Goldman Sachs had gained EU regulatory approval.The sale of the remainder of Candover’s assets could still take years to complete, with the firm insisting there will be no fire-sale.GERRY GRIMSTONECANDOVERCHARISMATIC chairman Gerry Grimstone will soon sever his relationship with Candover that has lasted over a decade. The 60-year-old announced yesterday he will step down when the sale of Candover Partners Limited to a group of its executives closes next year. His time at the firm has been tumultuous – for many years Candover was a roaring success story, wielding a Midas touch that was the envy of the private equity industry.But Grimstone experienced the flip side of the coin when the financial crisis hit and the value of its portfolio plummeted. He received criticism for a number of acquisitions at the firm that were deemed ill-judged.Grimstone, though, is no stranger to criticism. He came under fire from the left for his role in the privatisation drive of the early 1980s.As assistant secretary at the Treasury from 1982 to 1986, he was a key adviser to Margaret Thatcher during her landmark £5.4bn asset sales. He oversaw 20 major sell-offs including offloading the water companies and privatising BT. While the sales were lauded in the City, it was famously described as “selling off the family silver” by Harold Macmillan. He later went on to advise the Labour government as it attempted to claw back funds during the financial crisis by selling off state assets.While he made his name as a civil servant, he made his fortune as an investment banker. He joined Schroders in 1986, where he rose to vice chairman of worldwide investment banking, splitting his time between London, Hong Kong and New York.He maintains an interest in the civil service, holding the position of chairman of the Nominations Committee and member of the Remuneration Committee. He was overlooked for the chairmanship of the court of directors at the Bank of England.The physically imposing Grimstone has a reputation for being both intelligent and combative.The divorcee with three children has also been a member of the Standard Life board since 2003. by Taboolaby TaboolaSponsored LinksSponsored LinksPromoted LinksPromoted LinksYou May LikeMisterStoryWoman Files For Divorce After Seeing This Photo – Can You See Why?MisterStoryTotal PastThe Ingenious Reason There Are No Mosquitoes At Disney WorldTotal PastSerendipity TimesInside Coco Chanel’s Eerily Abandoned Mansion Frozen In TimeSerendipity TimesPeople TodayNewborn’s Strange Behavior Troubles Mom, 40 Years Later She Finds The Reason Behind ItPeople TodayBetterBe20 Stunning Female AthletesBetterBemoneycougar.comThis Proves The Osmonds Weren’t So Innocentmoneycougar.comZen HeraldNASA’s Voyager 2 Has Entered Deep Space – And It Brought Scientists To Their KneesZen HeraldAlphaCute30 Rules That All “Hells Angels” Have To FollowAlphaCuteDefinitionDesi Arnaz Kept This Hidden Throughout The Filming of ‘I Love Lucy’Definition KCS-content whatsapp Tags: NULL Candover to sell CPL to its management More From Our Partners Biden received funds from top Russia lobbyist before Nord Stream 2 giveawaynypost.comRussell Wilson, AOC among many voicing support for Naomi Osakacbsnews.comKiller drone ‘hunted down a human target’ without being told tonypost.comBrave 7-Year-old Boy Swims an Hour to Rescue His Dad and Little Sistergoodnewsnetwork.org980-foot skyscraper sways in China, prompting panic and evacuationsnypost.comPolice Capture Elusive Tiger Poacher After 20 Years of Pursuing the Huntergoodnewsnetwork.orgA ProPublica investigation has caused outrage in the U.S. this weekvaluewalk.comAstounding Fossil Discovery in California After Man Looks Closelygoodnewsnetwork.orgNative American Tribe Gets Back Sacred Island Taken 160 Years Agogoodnewsnetwork.orgcenter_img Show Comments ▼ Monday 6 December 2010 9:00 pm Share whatsapplast_img read more

CIC Insurance Group (CIC.ke) 2014 Abridged Report

first_imgCIC Insurance Group (CIC.ke) listed on the Nairobi Securities Exchange under the Insurance sector has released it’s 2014 abridged results.For more information about CIC Insurance Group (CIC.ke) reports, abridged reports, interim earnings results and earnings presentations, visit the CIC Insurance Group (CIC.ke) company page on AfricanFinancials.Document: CIC Insurance Group (CIC.ke)  2014 abridged results.Company ProfileCIC Insurance Group is a leading insurance company offering products and services for general and life insurance through operations in Kenya, Sudan and Uganda. The company also provides solutions for fund and asset management and private equity investment. Its product offering covers needs relating to motor, marine, agriculture, personal accident, school products, customised products, travel, domestic package, property, theft, fire and consequential loss and sports injury insurance. Its life assurance division covers areas ranging from group life and keyman plans to family protection, loan guard insurance, pension, board member and universal endowment plans. CIC Insurance Group has expertise in corporate, family, health and asset management as well as equity, balanced, fixed income and money market fun management. The company invests in equity and government securities, properties and loans and serves retail and corporate entities. Founded in 1968 and formerly known as The Co-Operative Insurance Company of Kenya, the company changed its name to CIC Insurance Group in 2010. Its head office is in Nairobi, Kenya. CIC Insurance Group is listed on the Nairobi Securities Exchangelast_img read more

What’s inside Rugby World’s autumn Tests special

first_img TAGS: Highlight What’s inside Rugby World’s autumn Tests specialThe autumn Tests are upon us and the December 2018 edition of Rugby World is the perfect off-field accompaniment to the world-class on-field action. We’ve even produced FOUR covers to celebrate the upcoming Internationals.It is packed with big-name interviews and expert analysis of the game’s major talking points. Here are 15 reasons to pick up Rugby World’s December 2018 edition…1. New Zealand’s Brodie RetallickWith the All Blacks touring Europe, former England fly-half Stuart Barnes explains why he thinks Brodie Retallick is the best player in the world and how he has redefined the second-row role.2. England’s Jonny MayHe’s not the biggest wing at Eddie Jones’s disposal but Jonny May is England’s most attacking threat. The Leicester Tiger discusses size and why he thinks players’ careers will become shorter.Danger man: Jonny May on the attack for England (Getty Images)3. South Africa Does South Africa hold the key to rugby’s future? The country’s ability to be part of both northern and southern hemisphere competitions could alleviate concerns over the sport’s financial sustainability.4. Team GB SevensWith this season’s World Sevens Series acting as a qualifier for the 2020 Olympics, Stephen Jones says the home unions need to put a proper Team GB structure in place for sevens to succeed in Britain.5. Ireland’s Rob Kearney                               The full-back is Irish rugby’s most decorated player and he has plenty of off-field business interests too. He covers both topics in this interview.Keeping busy: Ireland full-back Rob Kearney at his Ballsbridge pub (Inpho)6. Japan 2019Rich Freeman reports from Japan on the inspiring story of Kamaishi and assesses how the host nation is shaping up for the World Cup on and off the field.7. Repechage lowdownThe final qualifier for the 2019 World Cup will be decided in Marseille in November – and we have all the info you need. Plus, Brett Beukeboom talks about Canada’s last shot at reaching Japan.DOWNLOAD RUGBY WORLD’S DIGITAL EDITION HERE8. Scotland’s Greig Laidlaw The December 2018 edition of Rugby World magazine is packed with big-name interviews and expert analysis ­– find out more here Hot topic: All Blacks perform the haka in Yokohama (Getty Images)PLUS, THERE’S ALL THIS…Inside the Mind of… Harry WilliamsWhat it’s like to… Go viral at the Olympics, by Brazil’s Izzy CerulloGlasgow and Scotland fly-half Adam HastingsThe new TMO trial explainedClub Hero – Ulster centre Darren CaveRising Stars Dan Davis and Caelan DorisLeigh Halfpenny’s goalkicking tipsGrass-roots club news in Club FocusSouth Africa Sevens star Chris DryJasmine Joyce on an exciting autumn series for Wales WomenFollow Rugby World on Facebook, Twitter and Instagram. Centre point: Jonathan Davies has been in decent form for the Scarlets (Huw Evans Agency)12. Georgia’s Merab Sharikadze“We won’t grow if we don’t get opportunities.” The centre explains why Georgia’s progress could stall without better fixture.13. Ben Ryan’s World Cup squadThe gold medal-winning coach turns selector and picks his 31-man England squad for next year’s World Cup – be prepared for bombshells!14. Cory HillThe Dragons and Wales lock talks Liverpool, lineouts and leading men.15. Should the haka be banned from rugby?Former Ireland coach Eddie O’Sullivan and ex-All Blacks prop Ben Franks present different sides of the argument.  LATEST RUGBY WORLD MAGAZINE SUBSCRIPTION DEALS “I don’t mind being written off. I enjoy it actually.” The Scotland scrum-half talks about competition, Clermont and the World Cup.Highlight: Greig Laidlaw celebrates beating England earlier this year (Inpho)9. Argentina’s Bautista Delguy“Super Rugby fits my game. I like it fast and open.” The Pumas wing gives us an insight into his background and assesses his breakthrough season.10. Australia AnalysedSean ‘The Analyst’ Holley looks at the three areas the Wallabies are vulnerable – and offers solutions to their game’s deficiencies.11. Wales’ Jonathan DaviesThe centre is back from a long-term injury lay-off and in our ‘Downtime’ Q&A covers guilty pleasures, dinner guests and Ryan Giggs.last_img read more

Japan 2019 Transport Guide: JR West Japan

first_imgThe Foodie The Seto Inland Sea is known for its fresh seafood – conger eel and octopus among the favourites.Hiroshima is also Japan’s biggest producer of oysters, so they are a must-try when in the area. There are various ways to enjoy them – fried, steamed, grilled, raw or with miso (kaki-no-dote-nabe).The Adventurer Head to Miyajima, a small island in Hiroshima Bay. This provides one of Japan’s top three scenic views and the island is home to the Itsukushima Shrine, a World Heritage Site with a ‘floating’ torii gate. There are plenty of hiking trails from which you can explore the island too.The Rugby World Cup also coincides with the autumn dates (28 September to 4 November) of the Setouchi Triennale art festival. The theme this year is Restoration of the Sea and you can reach the main venue, Naoshima Island, by train and ferry in only one-and-a-half hours from Okayama.Neon streets: Nagarekawa has great nightlife (Image is for illustration purposes)The Party Animal Floating view: Okayama’s Korakuen Garden TAGS: Japan LATEST RUGBY WORLD MAGAZINE SUBSCRIPTION DEALS Take a bullet train to discover the area near the Seto Inland Sea For the best nightlife, head to Nagarekawa in Hiroshima. Once a creative centre for artists and writers, Nagarekawa’s narrow streets, which are something of a maze to traverse, are now full of food and drink establishments. There’s even an Irish pub, Molly Malone’s!Other top bars are the Ofuku Kimono & Sake Bar, where you can not only try local dishes and drinks but be fitted with a kimono, and Tropical Bar Revolucion, which is renowned for its cocktails. Advertising FeatureJapan 2019 Transport Guide: JR West JapanFrom the rugby venues in Kobe and Osaka or Tokyo and Yokohama, you can reach the Setouchi area by shinkansen…THE TICKETSA JR-West Setouchi Area Rail Pass is a great option if you plan to travel around the Seto Inland Sea region by train. It covers five days and you can purchase it from travel agents outside Japan or in the region itself. Many other rail passes are available. See westjr.co.jp/global/en/ticketThe Culture Vulture Okayama’s Korakuen Garden is rated one of Japan’s three best gardens and dates back 300 years. Tie in a stroll there with a visit to Okayama Castle, also situated on the Asahi River.The Bikan Historical Area in Kurashiki is the most picturesque merchants’ quarter in Japan and you can take a traditional boat trip down the canal.Historical site: Hiroshima Peace Memorial ((c) Hiroshima Pref.)In Hiroshima, you’ll find the Atomic Bomb Dome, which remains as a reminder of the 1945 attack. You can also visit the Hiroshima Peace Memorial Museum, opened in 1955 to advocate world peace while charting the build-up and aftermath of the bombing.last_img read more

Beginning with the end in mind: Results-based accountability training is a…

first_img Please enter your name here Please enter your comment! You have entered an incorrect email address! Please enter your email address here LEAVE A REPLY Cancel reply TAGSOrange County Commissioner Rod Love Previous articleSonny’s BBQ Commits to Fourth Sponsorship with Habitat for HumanityNext articleApopka Burglary Report Denise Connell RELATED ARTICLESMORE FROM AUTHOR Share on Facebook Tweet on Twitter From District 2 Orange County Commissioner Rod LoveLast week, District 2, in partnership with Bethune-Cookman University of Daytona Beach, Florida Memorial University of Miami Gardens, Edward Waters College of Jacksonville and Florida Agricultural and Mechanical University (FAMU) of Tallahassee, hosted a training and seminar for Results-Based Accountability (RBA) principles and concepts. Historically Black Colleges and University researchers lead by Bethune-Cookman University were present to direct and facilitate the workshop with the funding support of the Annie E. Casey Foundation. Before delving into what took place at the training, as always, I would like to express my sincere appreciation and gratitude to all those who made this workshop possible. I would like to thank Dr. Randy Nelson, Ph.D. and the University researchers, Dr. Kideste Mariam Usef, Ph.D. and Dr. Nichole Jones, Ed.D., LMHC, QS with Bethune-Cookman University; Dr. Felecia Dix-Richardson, Ph.D., with FAMU; Dr. Sylvia Boynton, Ph.D., LCSW, LMFT, QCS, CCMS, of Florida Memorial University; Dr. Kimberly Davis, Ph.D., of Edward Waters College; Charter Spectrum for the Public Service Announcement aired prior to the training; the Central Florida Hotel and Lodging Association (CFHLA); the Downtown Crowne Plaza for hosting the venue for the training; Orange County staff; City of Apopka Commissioner Alexander Smith and Paula Hosington from Mayor-Elect Jerry Demings Transition Team for attendance; and all individuals and entities that attended.District 2 Orange County Commissioner Rod LoveThis Performance Management, Measurement, and Accountability workshop was specifically geared toward public and private human service professionals, providers, and funders. Individuals from various organizations and agencies were in attendance, including Community Based Care of Central Florida, Department of Juvenile Justice, and Strengthening Our Sons, to name a few. Recent discussions and commitments by Orange County Government to increase the level of funding for youth services in Orange County made the timing of this workshop ideal. The workshop focused on defining the desired results and conditions to be achieved relative to youth and family services; identifying key indicators and performance measures to determine whether the desired results are achieved and developing an action plan to achieve and/or improve upon the desired results.A performance management tool known as Results Based Accountability (RBA) was introduced to attendees and used as the foundational pillar of the workshop’s focus. RBA is a disciplined way of thinking and acting to improve entrenched and complex social problems. Communities use RBA to improve the lives of children, youth, and families and is also used by organizations to measure and/or improve the effectiveness of their programs. Developed by Mark Friedman and described in his book Trying Hard is Not Good Enough (2005), RBA is being used in all 50 states in the U.S. and more than a dozen countries around the world to make measurable changes in lives, communities, and organizations.Following a pre-test and introduction on RBA, Dr. Nelson discussed indicators, performance measurements, baseline/trend line, data, and partnerships. To conclude this section of the workshop, attendees participated in a post-test to measure how much they had learned about RBA.To really begin applying what they had learned from the workshop, attendees were asked to develop a result statement for their agency/organization; discuss strategies to implement RBA within their agency/organization, and to identify potential barriers and challenges with implementing RBA within their agency or organization.At the conclusion of the workshop, Dr. Nelson and Commissioner Smith offered their thoughts on how they think the workshop went and what needs to be done moving forward.“This is just an initial conversation,” said Dr. Randy Nelson. “But it all starts with this conversation.”“It was exciting for me to come today to listen, see and observe what took place,” Said Commissioner Alexander Smith. “By getting this many different organizations together in one place at the same time, this lets us know what each individual and organization has and can bring to the table and work together cooperatively to get good results.”It was my hope that everyone in attendance at the workshop left with a basic understanding of RBA principles and concepts as well as have the tools to identify strategies to incorporate the RBA framework across various human service sectors. Within 24-48 hours, myself and Dr. Nelson were already receiving feedback on the workshop and even requests to hold additional workshops.This workshop was streamed live and can be found on my Facebook page at Orange County District 2 Commissioner. The Anatomy of Fear Support conservation and fish with NEW Florida specialty license plate Save my name, email, and website in this browser for the next time I comment. Free webinar for job seekers on best interview answers, hosted by Goodwill June 11 last_img read more

Two charity banner ads top the top 10 league

Two charity banner ads top the top 10 league  11 total views,  1 views today AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to LinkedInLinkedInShare to EmailEmailShare to WhatsAppWhatsAppShare to MessengerMessengerShare to MoreAddThis AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to LinkedInLinkedInShare to EmailEmailShare to WhatsAppWhatsAppShare to MessengerMessengerShare to MoreAddThis The ads topped the list presumably because some or all of the media space was donated. As a result the ads secure more coverage than those from Norwich Union, the Department of Health, Deutsche Bank and KLM.> >> >> .>>>> ——————————————————————– Howard Lake | 23 November 2000 | News Two charity banner ads have topped the top 10 league of ad campaigns.Two charity banner ads have topped the top 10 league of ad campaigns Banner adverts from Macmillan Cancer Relief and the Royal British Legion have topped this week’s list of online ad campaigns in order of estimated spend. The Macmillan ad is a direct fundraising advert, suggesting that you “click here to donate one pound to Macmillan…”. The Legion advert encourages you to “click here to join the 2-minute silence.” Advertisement About Howard Lake Howard Lake is a digital fundraising entrepreneur. Publisher of UK Fundraising, the world’s first web resource for professional fundraisers, since 1994. Trainer and consultant in digital fundraising. Founder of Fundraising Camp and co-founder of GoodJobs.org.uk. Researching massive growth in giving. read more

Paypal launches UK version

first_img About Howard Lake Howard Lake is a digital fundraising entrepreneur. Publisher of UK Fundraising, the world’s first web resource for professional fundraisers, since 1994. Trainer and consultant in digital fundraising. Founder of Fundraising Camp and co-founder of GoodJobs.org.uk. Researching massive growth in giving.  55 total views,  1 views today Advertisement Howard Lake | 16 October 2003 | News AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to LinkedInLinkedInShare to EmailEmailShare to WhatsAppWhatsAppShare to MessengerMessengerShare to MoreAddThis You too could add a donate button to your Web site like the one above, or include donate text or graphic links in your e-mail messages or e-mail newsletters.First though you need to register with Paypal. Don’t be put off by the use of US dollar signs on the first page: you can sign up via this page for the UK programme. Tagged with: Digital Finance Paypal launches UK version  56 total views,  2 views today AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to LinkedInLinkedInShare to EmailEmailShare to WhatsAppWhatsAppShare to MessengerMessengerShare to MoreAddThis PayPal UKOnline payment facility Paypal now has a full UK version, allowing individuals and organisations to make and more importantly receive payments and donations in a choice of currencies including pound sterling and euro.Paypal is a popular online payment method in the USA and is used extensively by people in the UK who use online auction sites. As such it represents a useful additional method of accepting payments via the Web.Indeed, the new UK version can allow charities or indeed anyone with an account to accept payment from a credit or debit card or bank account via the Web. As well as straight payments it includes the option of encouraging donations, as well as subscriptions or regular payments. It does not however include a Gift Aid reclaim option.Paypal’s fees are fairly low. There is no charge to open an account, send money, or add funds to your account. Withdrawals of £50 or more are now free, and withdrawals of less than £50 cost 25p. Paypal’s standard rate for handling payments/donations is 3.4% plus 20p but if you qualify for a merchant rate this can drop to 2.7% plus 20p. If you receive a payment from a user in another country, an additional cross border fee will be charged.Paypal’s UK operations include a new European customer service facility based in Dublin.If you have not used Paypal before it is well worth exploring now it has a UK version. You can find out what it is like to make a donation online using the system by clicking on the button below. UK Fundraising will be testing accepting donations from users of this site and its e-mail newsletter who would like to express their gratitude for the free service we provide.last_img read more

BHF partners with George Foreman grill range

first_img AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to LinkedInLinkedInShare to EmailEmailShare to WhatsAppWhatsAppShare to MessengerMessengerShare to MoreAddThis Howard Lake | 2 August 2006 | News The British Heart Foundation (BHF) has partnered with the health grills promoted by former world heavyweight boxing champion George Foreman in a promotion that could raise £300,000 for the charity.The heart charity’s logo and campaign details will appear across the the range of George Foreman health grills’ packaging.The partnership will include a visit to the UK by George Foreman in October, followed by a TV advertising campaign. Advertisement BHF partners with George Foreman grill range  22 total views,  1 views today AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to LinkedInLinkedInShare to EmailEmailShare to WhatsAppWhatsAppShare to MessengerMessengerShare to MoreAddThiscenter_img Tagged with: corporate Recruitment / people About Howard Lake Howard Lake is a digital fundraising entrepreneur. Publisher of UK Fundraising, the world’s first web resource for professional fundraisers, since 1994. Trainer and consultant in digital fundraising. Founder of Fundraising Camp and co-founder of GoodJobs.org.uk. Researching massive growth in giving. Over 10 million George Foreman grills have been sold in the UK in the past five years, and over 80 million around the world.last_img read more

Fundraisers worried about job security and bullying

first_imgFundraisers worried about job security and bullying Tagged with: Research / statistics Howard Lake | 16 July 2010 | News AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to LinkedInLinkedInShare to EmailEmailShare to WhatsAppWhatsAppShare to MessengerMessengerShare to MoreAddThis  21 total views,  1 views today AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to LinkedInLinkedInShare to EmailEmailShare to WhatsAppWhatsAppShare to MessengerMessengerShare to MoreAddThis Fundraisers are feeling undervalued, worrying about their jobs and more are reporting being bullied, according to a survey.Only 42% of fundraisers are not concerned about their jobs compared to 49% last year and 14% report they have been bulled in the last 12 months – up 2% on last year. Generally among all charity staff morale is lower and workers are less engaged with their organisations.The Charity Pulse 2010 – Voluntary Sector Staff Satisfaction Survey conducted by Birdsong Charity Consulting and Third Sector also highlighted a number of areas where fundraisers’ opinions of people management have declined since the recession began. Only just over half (51%0 feel they get the support they need to do their job well, down 14% from three years ago. They feel less appreciated and are less happy with the way that poor performance is handled. There are fewer opportunities for personal development at charities with only 43% thinking their organisations offer a good level of development.This year respondents were asked which aspects of organisational culture are most important. Over all charity staff the most important aspects were: well-managed and professional; respects and values people; gets things done. Fundraisers, however, also disproportionately value cultures that are: inspiring and exciting; entrepreneurial; fun. Fundraising managers also prefer a culture that is challenging, whereas other fundraisers tend to attach more importance to an environment that is calm and relaxed and free from fear and blame.There is more information available on the survey at www.bird-song.co.uk About Howard Lake Howard Lake is a digital fundraising entrepreneur. Publisher of UK Fundraising, the world’s first web resource for professional fundraisers, since 1994. Trainer and consultant in digital fundraising. Founder of Fundraising Camp and co-founder of GoodJobs.org.uk. Researching massive growth in giving.last_img read more

Tricking an Enzyme Into Making Better Insulin

first_img Community News Your email address will not be published. Required fields are marked * Community News Get our daily Pasadena newspaper in your email box. Free.Get all the latest Pasadena news, more than 10 fresh stories daily, 7 days a week at 7 a.m. SURF student Mary Boyajian working in the lab of chemical engineer David Tirrell. Credit: Seth HansenMary Boyajian, a junior majoring in chemical engineering at Caltech, spent her summer as a student in the Summer Undergraduate Research Fellowships (SURF) program trying to trick an enzyme. The enzyme, tRNA synthetase, has a very specific chemical target, and Boyajian wanted the enzyme to ease up a bit on its requirements so that it might also find acceptable a slightly altered version of the target. The work might sound esoteric, but it was Boyajian’s piece of a project with an end goal that could benefit millions: devising a faster-acting insulin-replacement therapy for the treatment of diabetes.A normally functioning pancreas keeps blood sugar within a narrow range by releasing large bursts of the hormone insulin after meals. Insulin helps cells absorb excess glucose and prevents the liver from producing additional sugar. In the case of diabetics, however, either the cells become resistant to the effects of insulin or the body simply cannot produce enough of the hormone, so additional insulin is needed.In the 1920s, insulin isolated from animals became the first insulin-replacement therapy for diabetics. Forty years later, scientists figured out how to make human insulin in the lab. However, that synthetic insulin behaves a bit differently in the body. For example, it tends to clump up and therefore takes a long time for the body to absorb.To improve the speed or ease of absorption, chemists have designed replacement therapies that are analogs of human insulin, made by substituting some of insulin’s building blocks, or amino acids, with other naturally occurring amino acids. However, there is room for improvement. For example, scientists would like to make therapies that kick in faster, last longer, and offer a longer shelf life.In all current insulin-replacement therapies, certain naturally occurring amino acids are swapped for other naturally occurring amino acids. But in the lab of David Tirrell, the Ross McCollum–William H. Corcoran Professor and professor of chemistry and chemical engineering at Caltech, chemists are working with what are known as noncanonical amino acids. These variants are designed and made in the lab to have slightly altered chemical structures. If expressed in a protein, these synthetic amino acids can introduce entirely new functions or capabilities. Tirrell’s group has the idea to swap out a naturally occurring amino acid from insulin with a noncanonical amino acid to create a replacement therapy that would outperform those on the market today.Boyajian’s role this summer was to introduce specific mutations in the enzyme tRNA synthetase. Each of the 20 amino acids that are expressed naturally in proteins has its own tRNA synthetase that hunts within cells for its specific amino acid target, so that the amino acid can be incorporated in the right sequence to make proteins. Even a small difference in an amino acid’s structure will deter its tRNA synthetase.“When I started this project, I had no idea that changing one amino acid could change so much about a protein, but it can,” says Boyajian. “My job is to mutate the tRNA synthetase so that it won’t see a modified amino acid—one of our noncanonical amino acids—and say, ‘That’s the wrong one. Take it out.’”To get an idea of how she might mutate the enzyme, Boyajian studied the known structures of similar tRNA synthetases and how they interact with their target molecules.Once she had an idea for a mutation, she introduced the changes into the gene that codes for the tRNA synthetase. Then she used a standard technique in molecular biology called polymerase chain reaction (PCR) to make many copies of it. Next she grew cells with the mutated enzymes on media lacking the naturally occurring amino acid—think of it as a type of food for cells. Once the cells ate up any small traces of the amino acid in the media, she fed them one of the noncanonical amino acids. If a mutated enzyme worked, it was able to “eat” the new amino acids; if not, the cells eventually died.At the end of the summer, one of Boyajian’s mutated tRNA synthetases showed promising results in terms of incorporating one of the noncanonical amino acids, and she is now working to scale-up the size of cultures to determine whether the new enzyme can be used to produce proteins for future experiments. In the long term, if the enzyme is found to efficiently incorporate a specific noncanonical amino acid, the Tirrell lab would use the enzyme to produce novel insulins that could be assessed as potential biopharmaceuticals to improve the quality of life for patients.Boyajian, who also plays basketball and serves as one of the captains of the water polo team, says she learned a lot from her SURF experience. “My grad student mentors, Seth Lieblich and Kat Fang, were great, and everybody in the lab was very welcoming,” she says. “It’s really nice to see everything you learned in the classroom being applied.” Name (required)  Mail (required) (not be published)  Website  0 commentsShareShareTweetSharePin it Subscribe More Cool Stuff HerbeautyWhat Is It That Actually Makes French Women So Admirable?HerbeautyHerbeautyHerbeauty10 Most Influential Women In HistoryHerbeautyHerbeautyHerbeauty6 Strong Female TV Characters Who Deserve To Have A SpinoffHerbeautyHerbeautyHerbeauty10 Ways To Power Yourself As A WomanHerbeautyHerbeautyHerbeauty10 Special Beauty Tips That Make Indian Women So BeautifulHerbeautyHerbeautyHerbeauty7 Most Startling Movie Moments We Didn’t Realize Were InsensitiveHerbeautyHerbeauty EVENTS & ENTERTAINMENT | FOOD & DRINK | THE ARTS | REAL ESTATE | HOME & GARDEN | WELLNESS | SOCIAL SCENE | GETAWAYS | PARENTS & KIDScenter_img First Heatwave Expected Next Week Make a comment Top of the News faithfernandez More » ShareTweetShare on Google+Pin on PinterestSend with WhatsApp,Virtual Schools PasadenaHomes Solve Community/Gov/Pub SafetyCitizen Service CenterPASADENA EVENTS & ACTIVITIES CALENDARClick here for Movie Showtimes Science and Technology Tricking an Enzyme Into Making Better Insulin By KIMM FESENMAIER Published on Tuesday, December 1, 2015 | 11:00 am Pasadena’s ‘626 Day’ Aims to Celebrate City, Boost Local Economy Pasadena Will Allow Vaccinated People to Go Without Masks in Most Settings Starting on Tuesday Business News Home of the Week: Unique Pasadena Home Located on Madeline Drive, Pasadenalast_img read more